Product Name :
Supervillin Rabbit Polyclonal Antibody
Predicted band size :
Observed band size :
Synonyms:
Archvillin antibody p205/p250 antibody
Function :
Forms a high-affinity link between the actin cytoskeleton and the membrane. Isoform 1 (archvillin) is among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation. Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function. Isoform 2 may be involved in modulation of focal adhesions. Supervillin-mediated down-regulation of focal adehesions involves binding to TRIP6 (By similarity).
Antibody Type:
Rabbit Polyclonal Antibody
Immunogen :
KLH conjugated synthetic peptide derived from human Supervillin 51-150/2214
Species reactivity:
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep
Verify application:
ELISA, IHC-P, IF, ICC
Coupling :
unconjugated
Clone number:
RRID:
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at -20℃ for one year.Buy2-Aminoimidazole Avoid repeated freeze/thaw cycles.Fmoc-Ser-OtBu web The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃.PMID:33406909 When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage buffer solution:
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
1:5000-10000
Molecular weight :
248 KDa
Positive control :
Expressed in many tissues. Most abundant in muscle, bone marrow, thyroid gland and salivary gland. Isoform 1 (archvillin) is muscle specific.